Figure 4. SUVH9 and SUVH2 are required for establishment of DNA methylation and silencing at FWA.
A–D. Flowering time distributions shown as total leaf number of T1 populations of untransformed (light gray) or FWA transformed (black) plants of the indicated genotype. X-axes show total number of leaves at flowering time, and the Y-axes show the percentage of plants with the given leaf number. E. Bisulfite sequencing results of the FWA transgenes of late-flowering T1 plants. Black, CG methylation; gray, CHG methylation; white, CHH methylation. F. siRNA accumulation in various mutant backgrounds. MicroRNA 159 (mi159) was used as a loading control for SDC (siRNAs hybridizing to the tandem repeats) and 5S siRNAs.
