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. 2008 Nov 28;4(11):e1000280. doi: 10.1371/journal.pgen.1000280

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Johnson et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Mobility shift assays using either GST-SUVH2-SRA (SUVH2) or GST-SUVH9-SRA (SUVH9) and either unmethylated CG oligonucleotide (^uCG), methylated CG (^mCG), methylated CHG (^mCHG) or methylated CHH (^mCHH) as probe. B. Upper panel: Quantitative RT-PCR of the SDC gene relative to ACTIN was measured in suvh9 suvh2 kyp lines containing the following stable transgenes: myc-tagged SUVH9 (SUVH9) or myc-tagged SUVH9-SRA mutant (S252F; SUVH9^SRA). Lower panel: Western blot of transgenic plant extracts probed with myc antibody. C. Upper panel: Complementation of suvh2 was tested by MEA-ISR Southern blot using MspI digested DNA isolated from suvh2 lines containing the following stable transgenes: HA-tagged SUVH2 or HA-tagged SUVH2-SRA mutant (E262K; SUVH2^SRA). Lower Panel: Western blot of immunoprecipitated protein from suvh2 transgenic plants using HA antibody as probe.