Figure 1. BM-PC express Notch pathway ligands/receptors and show increased expression of notch downstream targets during endothelial differentiation.

A. Expression of Notch receptors and ligands in BM-PC was detected by RT-PCR. B. Expression of Notch downstream targets (Hes 1, Hey 1 and 2) was detected at different time points during BM-PC endothelial differentiation by quantitative real-time PCR. C. Representative images (×200) of BM-PC at day 20 of culture showing positivity for endothelial lineage specific markers, acetylated LDL, CD31 , Flk-1 and vWF with DAPI nuclear counterstaining in blue. D. Quantification of BM-PC positive cells for acetylated LDL, CD31 , Flk-1 and VWF after 20 days of culture. Each experiment was performed in triplicate and the mean presented (n = 3).