Skip to main content
. 2008 Nov 18;3(11):e3752. doi: 10.1371/journal.pone.0003752

Figure 2. Notch pathway early inhibition impairs BM-PC adhesion and spreading to extracellular matrix, reducing the number of mature cells obtained at the end of the differentiation.

Figure 2

A. Expression of Hes 1 and Hey 2 72 h after GSI (10 uM) treatment was detected by RT-PCR. B. Quantification of adherent BM-PC 72 h after treatment with DMSO, GSI at 10 ηM or 10 µM, on 2% gelatin coated wells. C. Quantification of adherent BM-PC 48 h after treatment with DMSO and GSI at 10 µM, on 2% gelatin, fibronectin, collagen or laminin coated wells. D. Quantification of control or GSI BM-PC expressing double EC – lineage specific markers (acLDL/FLK-1 or acLDL/VWF) after 20 days of endothelial differentiation. E. Representative image (100×) of adherent cells under the different conditions. *P<0,05, **P<0,01. Each experiment was performed in triplicate and the mean presented (n = 3).