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. 2008 Nov 18;3(11):e3752. doi: 10.1371/journal.pone.0003752

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Caiado et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Quantitative analysis of Matrigel-induced tube branching of HUVEC untreated or GSI-treated monoculture and co-cultured with control and GSI-treated BM-PC. Results show the average number of branch points in 5 high power fields. B. Representative images of HUVEC tube formation in monoculture and co-cultured with control and GSI treated BM-PC. Phase contrast microscopy (original magnification, 40×). C. Quantitative analysis of Matrigel-induced tube branching of HUVEC untreated or GSI-treated monoculture and co-cultured with control and GSI-treated BM-PC, using different doses of GSI treatment. D. Representative images of HUVEC tube structures in the presence of control or GSI BM-PC acetylated LDL-FITC labelled (original magnification, 40×). Arrows identify acetylated LDL-FITC labelled control or GSI BM-PC. E. Quantification of control or GSI BM-PC found in or out of endothelial tubular structures. Results expressed relatively to the total number of BM-PC counted. *P<0.05. Each experiment was performed in triplicate and the mean presented (n = 3).