Table 1.
Sample | Activity, pmol·min−1·µg−1 | CEL Concentration, ng CEL/µg whey protein |
---|---|---|
Activity was measured by the release of [14C]oleate from cholesteryl-[14C]oleate in the presence of bile salt and BSA. Calculation of the mass of carboxyl ester lipase (CEL) present was based on comparison with the purified CEL standard. Values are averages of duplicates performed on 3 different concentrations of enzyme or whey protein. Protein was determined by Lowry assay. | ||
Purified CEL | 8,889 | |
Milk | ||
#1 | 65 | 7 |
#2 | 394 | 44 |
B6 | 145 | 16 |
2d | 596 | 67 |
14d | 277 | 31 |