Gradient gel: | 4% | 13% | Stacking gel: | 3.5% |
AA/bisAA 3 mix | 2 ml | 6.5 ml | 0.6 ml | |
Gel buffer 3´ | 8.3 ml | 8.3 ml | 2.7 ml | |
Glycerol | 5 g | |||
H2O | 14.7 ml | 5 ml | 4.7 ml | |
Total volume | 25 ml | 25 ml | 8 ml | |
10% APS | 139 µl | 125 µl | 67 µl | |
TEMED | 14 µl | 12.5 µl | 6.7 µl |