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. 2008 Jun 17;(16):724. doi: 10.3791/724
Gradient gel: 4% 13% Stacking gel: 3.5%
AA/bisAA 3 mix 2 ml 6.5 ml   0.6 ml
Gel buffer 3´ 8.3 ml 8.3 ml   2.7 ml
Glycerol   5 g    
H2O 14.7 ml 5 ml   4.7 ml
Total volume 25 ml 25 ml   8 ml
10% APS 139 µl 125 µl   67 µl
TEMED 14 µl 12.5 µl   6.7 µl