Figure 4. Proteome profiling of ER proteins at baseline.

The SILAC methodology followed by mass spectrometry of microsomal preparations was processed as described in Materials and Methods. Untreated labeled DDR1 cells were mixed at a 1∶1 ratio with unlabeled sh2B5 or sh2B5+2B5(R195H) cells. The level of Bip, PDIA1, PDIA3, PDIA4 and PDIA6 relative to DDR1 control cells is shown.