Figure 2. H-RasV12 induces cell cycle arrest in immortalized FHL2^−/− MEFs.

(A) Immortalized wt, FHL2^−/− and FHL2^−/−-restored MEFs were infected with pBabe-RasV12 or control pBabe retrovirus and selected for drug resistance. (B) All FHL2^−/− cells were positive in β-galactosidase assay due to the insertion of Lac Z cDNA at the FHL2 locus. (C) Ki-67 immunofluorescence analysis in vector- and H-RasV12-transduced FHL2^−/− MEFs. Nuclei were counterstained with DAPI. (D) Expression of Ras, p53, p21^CIP1, p16^INK4a and cyclin D1 in H-RasV12- (R) or vector- (V) transduced wt and FHL2^−/− cell lines. Equal amounts of protein extracts were analyzed by immunoblotting.