Figure 5. Reversal of the cell cycle arrest in H-RasV12-transduced FHL2^−/− MEFs.

(A) The indicated cell lines transduced by H-RasV12 or control vector are shown at cell cycle arrest stage (passage 0) and after reversal of the cell cycle arrest (passage 10). (B) Analysis of the p53 protein in cells at passage 10 after H-RasV12 transduction. Cells were treated with doxorubicine as described in Fig. 3A. (C) Western blot analysis of cell cycle regulators in wt and FHL2^−/− cell lines at passage 10 after transduction with H-RasV12 (R) or empty vector (V). (D) Growth curves of the indicated cell lines after reversal. Equal numbers of cells were initially plated in triplicate wells, and cells were counted at indicated times. Mean values±standard deviation from three independent experiments are shown. Data presented are representative of 3 independent H-RasV12- and vector-expressing MEF lines.