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. 2008 Nov 24;3(11):e3798. doi: 10.1371/journal.pone.0003798

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Western Blot analysis showing an inverse correlation between total levels of detectable Robo and growing amount of Comm protein in COS7 cells. Cells were transfected with 0, 0.03, 0.06, 0.120 and 0.250 micrograms of Comm-myc (upper two panels) or myc tagged Comm^LPAA (lower panel) plasmidic DNA and 0.5 micrograms of HA-tagged Robo, Robo^SD, Frazzled or Frazzled^CS. (B) Quantification of signal intensity of (A).