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. 2008 Nov 24;3(11):e3798. doi: 10.1371/journal.pone.0003798

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A–B) Confocal images of single cells expressing the fluorescent marker CD8GFP (green) and an HA-tagged version of either Robo or Robo^SD (red). Panel (A) shows the soma of poxn-GAL4 expressing cells at stage 13 of embryonic development, namely while their growth cone is crossing the midline; panel (B) shows analogous cells at stage 15, after the crossing is complete. Notice that while there is no difference between Robo and Robo^SD localization at stage 15 (B), a clear difference is observable at stage 13 (A). (C) Same experiment as in (B) but with ectopic expression of a uas-Comm transgene. (D) Lower magnification images of one segment of the CNS of UAS-HA-Robo (upper two) and UAS-HA-Robo^SD (lower two) expressing embryos, before crossing (leftmost two) and after crossing (rightmost two). Images in panels A–C are acquired using 100× magnification; images in panel D are acquired using 63× magnification. White size-bar indicates 10 µm.