Table II.

Second generation variant activity and stability

Variant no.	% of pH 4.5 activity relative to OPT	% of pH 6.0 activity relative to OPT	Time enzyme remains active in presence of pepsin and pH 4.5 buffer
10184	104	113	−
10185	82	111	++
10186	133	141	−
10187	115	113	+
10188	130	115	−
10189	113	135	++
10191	75	118	+
10192	80	101	−
10193	104	91	−
10194	139	162	+
10196	97	128	++
10197	120	122	−
10198	113	150	−
10200	109	117	−
10202	155	138	−
10203	118	159	++
10204	93	122	++
10205	70	113	+++
10206	78	113	++
10207	148	96	−
10208	214	150	−
10209	77	74	+
10210	73	99	+
10211	150	179	+
10212	152	131	−
10213	196	181	−
10214	117	112	+
10215	154	121	−
10216	198	127	−
10217	127	102	−
10218	73	79	−
10219	74	96	+++
10220	124	115	+
10221	126	131	+
10222	49	69	+
10223	88	82	−
10224	214	191	+
10225	107	110	−
10226	169	109	+
10227	154	102	−
10228	129	117	−
10229	106	97	+
10230	177	186	+
10231	200	190	+

The data are reported as described in the caption of Table I.