Figure 2.

Effects of hypoxia on src-family kinase tyr-416 auto-phosphorylation and protein tyrosine phosphorylation in intra-pulmonary artery. (A) Hypoxia enhanced phospho-src (tyr-416) immunoreactivity at 60 and 54 kDa in a time-dependent manner (*P < 0.05 vs. control, n = 9–11 rats). (B) Hypoxia (30 min) also enhanced phospho-tyrosine immunoreactivity at several protein bands (*P < 0.05, **P < 0.01 vs. control, n = 20 rats). At ∼120, ∼95, and ∼75 kDa, this enhancement was reversed by either PP2 or SU6656 (30 µM, ^†P < 0.001 vs. hypoxia alone, n = 8–11 rats). At ∼65 kDa, although not enhanced by hypoxia, basal immunoreactivity was also greatly inhibited by both inhibitors (^#P < 0.0001 vs. control).