Table 1.
The Cytomim system is cost-effective
| System | Reaction size (μl) | Energy source and nucleotides | Synthesis duration (h) | CAT produced (mg/ml) | Energy source and nucleotides cost (US$/ml reaction) | mg protein/ (US$ energy source and nucleotides) |
|---|---|---|---|---|---|---|
| PANOx | 15 | PEP, NTPs | 3 | 0.74±0.02 | 1.64 | 0.45 |
| PANOx | 15 | Pyruvate, NTPs | 3 | 0.06±0.02 | 0.52 | 0.12 |
| Cytomim | 15 | Pyruvate, NTPs | 6 | 0.73±0.04 | 0.52 | 1.40 |
| Cytomim | 15 | Glutamate, NTPs | 6 | 0.51±0.05 | 0.51 | 1.00 |
| Cytomim | 15 | Glutamate, phosphate, NTPs | 6 | 0.85±0.05 | 0.51 | 1.65 |
| Cytomim | 15 | Glutamate, phosphate, NMPs | 6 | 0.62±0.06 | 0.004 | 155 |
Relative product yields in Eppendorf batch reactors (mg protein/US$ energy source and nucleotides cost) demonstrate a significant increase for the new Cytomim system when NMPs and phosphate are used. Reagent prices are from manufacturer's online catalog in August 2008.