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. 2008 Oct 14;4:222. doi: 10.1038/msb.2008.61

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Copyright © 2008, EMBO and Nature Publishing Group

This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits distribution and reproduction in any medium, provided the original author and source are credited. Creation of derivative works is permitted but the resulting work may be distributed only under the same or similar licence to this one. This licence does not permit commercial exploitation without specific permission.

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Quantitative accuracy as a function of dwell time and cycle time. Quantification of a peptide using different settings for dwell time and cycle time. Reducing the dwell time from 50 to 5 ms decreases accuracy. With cycle times of 10 or 20 s, the peak height cannot be estimated correctly even though the accuracy of the individual data points is excellent at 500 ms dwell time. Changes in dwell time do not affect absolute signal intensity as it is plotted normalized as ‘counts per second'.