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Validation of transitions for yeast proteins. SRM-triggered MS/MS experiment for the validation of transitions for a high- (A) and a low-abundance (B) protein of S. cerevisiae. Owing to the low abundance of protein YKL141W (B), the SRM traces for the corresponding peptide AAIAEEQILNK were of low intensity. Nevertheless, the acquired MS/MS spectrum confirmed the identity of the peptide with high confidence.
