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. Author manuscript; available in PMC: 2009 Sep 15.

Published in final edited form as: Anal Biochem. 2008 Jun 5;380(2):315–322. doi: 10.1016/j.ab.2008.05.048

Simulation of amplification. (A) A simulated exponential amplification curve with E_max=1.9 (i) generated using an unconstrained efficiency T_n+1=T_n × 1.9 (T₀=1), (ii) efficiency limited as a simple function of increasing template T_n+1=T_n × (1.9−0.001×T_n ^0.5), and (iii) efficiency limited by a more physically realistic function T_n+1=T_n × (1.9−(0.001×T_n ^0.5 + 0.01×T_n ^0.25)). (B) Reaction efficiency as a function of cycle for the three models. (C) Log(fluorescence) of the exponential amplification.

Simulation of amplification. (A) A simulated exponential amplification curve with E_max=1.9 (i) generated using an unconstrained efficiency T_n+1=T_n × 1.9 (T₀=1), (ii) efficiency limited as a simple function of increasing template T_n+1=T_n × (1.9−0.001×T_n ^0.5), and (iii) efficiency limited by a more physically realistic function T_n+1=T_n × (1.9−(0.001×T_n ^0.5 + 0.01×T_n ^0.25)). (B) Reaction efficiency as a function of cycle for the three models. (C) Log(fluorescence) of the exponential amplification.