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. Author manuscript; available in PMC: 2009 Sep 15.

Published in final edited form as: Anal Biochem. 2008 Jun 5;380(2):315–322. doi: 10.1016/j.ab.2008.05.048

The user interface of KLAB-PCR. The window driven interface includes (i) data loading and analysis preferences (ii) E(max) identification through the first non-linear model (iii) data output including E_max, template estimation and model quality (iv) a graphical representation of template estimates from the first model (top green) final model (middle red) and C_t approaches (lower green) (v) PCR amplification curve (blue) generated from the final model and data curve (red) (vi) Log-linear fit (blue) to data (red) (vii) Progress of analysis. Replicates may be grouped based on sets of primers (A) or treated as a single group for example where the same reaction is replicated across 96 wells (B).

The user interface of KLAB-PCR. The window driven interface includes (i) data loading and analysis preferences (ii) E(max) identification through the first non-linear model (iii) data output including E_max, template estimation and model quality (iv) a graphical representation of template estimates from the first model (top green) final model (middle red) and C_t approaches (lower green) (v) PCR amplification curve (blue) generated from the final model and data curve (red) (vi) Log-linear fit (blue) to data (red) (vii) Progress of analysis. Replicates may be grouped based on sets of primers (A) or treated as a single group for example where the same reaction is replicated across 96 wells (B).