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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Anal Biochem. 2008 Jun 5;380(2):315–322. doi: 10.1016/j.ab.2008.05.048

Figure 7.

Figure 7

Determination of zygosity by comparison of transgene and endogenous template values. Serially diluted DNA isolated from single copy transgenic mice was subjected to PCR analysis with primer pairs Determination of Copy number. An endogenous region of the genome or a portion of the human transgene were targeted for PCR analysis. Data from separate reactions was combined to demonstrate the different ratios of relative template values of the templates indicative of a homozygous (A) or heterozygous (B) state.