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. Author manuscript; available in PMC: 2008 Nov 14.

Published in final edited form as: Biochemistry. 2006 Dec 12;45(49):14683–14694. doi: 10.1021/bi061159c

Phase transition behavior of C₃₂phytBAS.

A: DSC of C₃₂phytBAS dispersion (1 mg lipid/4 µL H₂O) in sealed stainless steel pans, heating rate of 1°C/min. One out of three trials is shown. The phase transition temperature (T_m = 14.3 °C) and transition enthalpy (−2.23 J/g) are displayed.

B: Temperature-dependent proton-decoupled ³¹P NMR spectra of C₃₂phytBAS in D₂O. Top to bottom: 30, 25, 20, 15, 12, 10, 5, 0, −5 °C. The lipid was hydrated (20 mg lipid/1 mL D₂O) and extruded through 200 nm track-etch membranes. Typically, 1600 scans were acquired with a relaxation delay of 1 s, 90° pulse of 6 µsec, acquisition time of 0.2 s. The spectra were processed with a line broadening of 200 Hz.

Phase transition behavior of C₃₂phytBAS.

A: DSC of C₃₂phytBAS dispersion (1 mg lipid/4 µL H₂O) in sealed stainless steel pans, heating rate of 1°C/min. One out of three trials is shown. The phase transition temperature (T_m = 14.3 °C) and transition enthalpy (−2.23 J/g) are displayed.

B: Temperature-dependent proton-decoupled ³¹P NMR spectra of C₃₂phytBAS in D₂O. Top to bottom: 30, 25, 20, 15, 12, 10, 5, 0, −5 °C. The lipid was hydrated (20 mg lipid/1 mL D₂O) and extruded through 200 nm track-etch membranes. Typically, 1600 scans were acquired with a relaxation delay of 1 s, 90° pulse of 6 µsec, acquisition time of 0.2 s. The spectra were processed with a line broadening of 200 Hz.