Table 1.
Mutant yeast strains that exhibited high fluorescence intensity and were selected by flow-cytometry analysis. The “Human Ortholog” column indicates yeast genes with known human orthologs.
| Strain and Pathway | Human Ortholog of Deleted Gene | Function of Deleted Gene |
|---|---|---|
| Ubiquitin- proteasome pathway | ||
| ubc7Δ | Yes | Ubiquitin conjugating enzyme |
| hul5Δ | Yes | Hect domain E3 ubiquitin-protein ligase |
| cue1Δ | No | Recruiting the ubiquitin-conjugating enzyme Ubc7p to the ER |
| ubx4Δ | No | UBX (ubiquitin regulatory X) domain-containing protein |
| ubp14Δ | Yes | Ubiquitin-specific protease |
| rpn10Δ | Yes | Non-ATPase base subunit of the 26S proteasome |
| Vesical trafficking | ||
| snc1Δ | Yes | v-SNARE involved in membrane fusion |
| syn8Δ | Yes | Endosomal SNARE related to mammalian syntaxin 8 |
| erv46Δ | Yes | Localized to COPII-coated vesicles; membrane fusion |
| prm9Δ | No | Involved in COPII binding |
| erp1Δ | Yes | Localized to COPII-coated vesicles |
| erp2Δ | Yes | Localized to COPII-coated vesicles |
| pex22Δ | No | Putative peroxisomal membrane protein |
| vps54Δ | No | Vacuolar Protein Sorting |
| snx4Δ | Yes | Sorting nexin |
| Chaperones | ||
| ssa1Δ | Yes | Member of heat shock protein 70 (hsp70) family |
| cne1Δ | Yes | Calnexin; integral membrane ER chaperone |
| Microtubule system | ||
| bud14Δ | No | Involved in bud-site selection; A regulator of dynein |
| ady3Δ | No | Interact with spindle pole body components |
| Vacuolar (lysosomal) protease | ||
| lap4Δ | Yes | Vacuolar aminopeptidase |
| Other | ||
| pca1Δ | Yes | Cadmium transporting P-type ATPase |
| Unknown | ||
| yml089cΔ | No | Unknown |
| yal045cΔ | No | Unknown |
| ydr026cΔ | Yes | Protein of unknown function that may interact with ribosomes |