Fig. 1.

Effect of a high concentration of glucose and acetylcholine (ACh) on [Ca²⁺]_i in a single mouse islet in culture. Glucose at 16.7 mM (G16.7, a) and ACh at 100 μM (b) were added to the mouse islet in the presence of 5.5 mM glucose (G5.5) at the time indicated by the bar. The change in [Ca²⁺]_i was monitored for 30 min using excitation at 340 and 380 nm and emission at 510 nm, and the bar indicates exposure to the indicated nucleotide. Each experiment was repeated 3 times