Fig. 2.

Effect of ATP, 2-MeSADP, and UDP on [Ca²⁺]_i in a single mouse islet and beta-TC6 cells in culture. ATP at 100 μM (a, d), 2-MeSADP at 100 μM (b, e), and UDP at 200 μM (c, f) were added to the mouse single islet (a–c) and beta-TC6 cells (d–f) in the presence of 5.5 mM glucose at the time indicated by the bar. In beta-TC6 cells, [Ca²⁺]_i rise occurring in each islet-like cluster composed of a few or several cells (9–10 clusters) is shown with overlapping different colors. The change in [Ca²⁺]_i was monitored for 20 min using excitation at 340 and 380 nm and emission at 510 nm, and the bar indicates exposure to the indicated nucleotide. Each experiment was repeated 7–15 times