Fig. 4.

Effect of the PLC inhibitor U73122 and the IP₃ receptor antagonist 2-APB on 2-MeSADP- or UDP-induced [Ca²⁺]_i rise in a single mouse islet in culture. U73122 at 2 μM (a, c) and 2-APB at 30 μM (b) or 10 μM (d) were added to the islet 20 min prior to the addition of the nucleotides. The change in [Ca²⁺]_i was monitored for 20 min using excitation at both 340 and 380 nm and emission at 510 nm, and the bar indicates exposure to the indicated nucleotide or the inhibitor. Each experiment was repeated 3–4 times