Fig. 5.
Effect of the PLC blocker U73122 and the IP3 receptor antagonist 2-APB on 2-MeSADP- or UDP-induced [Ca2+]i rise in beta-TC6 cells. Cells were incubated in the presence of either 2 μM U73122 (a, c) or 30 μM 2-APB (b, d) for 20 min prior to addition of the nucleotides. The results are expressed as a change in ratio (calculated by subtraction of a baseline value from a peak height value). Each bar represents the mean ± SE (n = 16–32). *P < 0.05 in comparison to control in the absence of nucleotides