Fig. 3.

P2Y₂ or P2Y₄ receptor activation increases ERK1/2 phosphorylation. BACCs were treated with increasing concentrations of nucleotides and analogs for 10 min. Blots are representative of three independent experiments performed in triplicate (n = 3); the upper band (phosphorylated or nonphosphorylated) is ERK1 = 44 kDa, and the lower band is ERK2 = 42 kDa. Blot intensities were measured with the Odyssey Imaging System; values are phosphorylated ERK2 intensity divided by total ERK2 intensity. Points on the graph represent mean ± standard error of the mean. Con (control) refers to results obtained with unstimulated cells