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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Cell Motil Cytoskeleton. 2008 Aug;65(8):626–640. doi: 10.1002/cm.20289

Figure 1. Klp5 and Klp6 plasmid constructs and dimerization models.

Figure 1

A) Diagrams of the major domains of Klp5p and Klp6p, cloned as Pk1- or GFP-tagged constructs and expressed under the regulation of the NMT41 S. pombe plasmid expression system (Forsburg, 1993). Each construct, name given on the left, was made with both klp5+ and klp6+, except the tail-Pk construct, which was only made with Klp5. The full-length construct was previously described and integrated (West, et al., 2001). B) Models of the potential dimerizations of various kinesin domain proteins (all shown with GFP) either with themselves or with the endogenously expressed, full length Klp5p or Klp6p (no GFP). In all cases, the Klp fragment could be from either Klp5p or Klp6p, and the endogenous protein could be either Klp5p or Klp6p.