Table 1.

Primers used to construct expression plasmids

5tail 5′A: gtcgtcgacgggtggtggttctgatcaggacgctgtaac

5tail-3′A: caggatccctattaggtggctttctcttcttcg (N)

5tail-3′B: caggatccaccaccggtggctttctcttcttcg (C)

5neck-tail-5′: gtcgtcgacgggtggtttaaggaacatgatcagtgtagatcg

5motor-5′: gagtcgtcgacgatgtcaagacagtcgtccattac

5motor-3′: caggatccaccacttaacacttcggttttgatattc

5motor-neck-3′: caggatccaccaccatttgattgcgatg

6tail-5′A: gtcgtcgacgggtggtatttcttctcgagaaattaaaatg

6tail-3′A: caggatccctataaagcattaggagtattctcagtccc (N)

6tail-3′B: caggatccaccaccagcattaggagtattctcagtccc (C)

6neck-tail-3′: gtcgtcgacgggtggtgtaagtgaatatgtacgcaccatttac

6motor-5′: gagtcgtcgacgatgtcaagacagtcgtccattac

6motor-3′: caggatccaccacttaacacttcggttttgatattc

6motor-neck-3′: caggatccaccaccatttgattgcgatg

6full5′: gggaatgaaagaagggtcttcaatttcc

6full3′: gggagtccaccagcattaggagtattctcagtcccgc