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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: Dev Biol. 2008 Apr 9;319(2):192–200. doi: 10.1016/j.ydbio.2008.03.039

Figure 4.

Figure 4

Lineage specific suppression of PI POMC cells in FGF3 morphants. 1–2 cell stage POMC-GFP/PRL-RFP double transgenic embryos were either injected with 0.4 ng of FGF3 MO (B) or vehicle (A) and then cultured in routine medium. At 56 hpf, pituitary POMC-GFP/PRL-RFP expression patterns were analyzed in live embryos using confocal microscopy. In FGF3 morphant, POMC-GFP expressing cells are detected within the rostral pars distalis but not in the pars intermedia, whereas PRL-RFP expression is intact. Ventral pituitary view under high magnification (63x), anterior to the left. WT, wild type; MO, FGF3 morphant. RPD, rostral pars distalis; PI, pars intermedia. Scale bar, 50 µm.