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. Author manuscript; available in PMC: 2008 Nov 17.
Published in final edited form as: Neuroendocrinology. 2008 Jan 23;88(1):35–42. doi: 10.1159/000XXXXXX

Fig. 1.

Fig. 1

Stimulation of ORL1 [35S]GTPγS binding in the medial preoptic area enriched plasma membrane. Concentration-response curves were determined for nociceptin as described in the Methods section. Nonspecific binding was determined using 10 μM cold GTPγ S and was subtracted from each data set. Each value represents the mean ± SEM of at least 4–6 independent experiments performed in triplicate. * Significant difference of 10 −7–10−5 nociceptin in the estradiol benzoate (EB) + progesterone (EB+P) group compared to the EB and oil values: p < 0.05.