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. Author manuscript; available in PMC: 2008 Nov 17.
Published in final edited form as: Mech Dev. 2006 Dec 8;124(3):218–229. doi: 10.1016/j.mod.2006.11.005

Fig. 4.

Fig. 4

Expression of insulin but not glucagon or Somatostatin, is lost following prodrug dependent β cell ablation. (A – C) Marker analysis on ins:nfsB-mCherry untreated control embryos or embryos treated with Met from 56hpf to 80hpf (D to F). In situ hybridization with riboprobes to detect the following transcripts: insulin (ins) (A, D); glucagon (gcg) (B, E). Immunofluorescence detection of Somatostatin protein, using confocal microscopy, (C, F). Presence of the NTR-mCherry fusion protein can be seen in the control embryo (C) as red fluorescence, but is lost following ablation (F).