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Permeabilization of live E. coli ML35 cells by RMAD-4(62–94), proRMAD-4(20–94), and (DE/NQ)-proRMAD-4(20–94). E. coli ML35 cells growing in log-phase were exposed to 6 μm peptide concentrations in the presence of ONPG at 37 °C (see “Experimental Procedures”). (DE/NQ)-proRMAD-4(20–94) (-○-) induces greater membrane permeabilization and ONPG conversion than proRMAD-4(20–94) (-▾-) but less than RMAD-4(62–94) (-•-).
