FIGURE 1.

Monitoring mitophagy using C-terminal GFP-tagged mitochondrial protein processing during amino acid starvation. Wild-type (WT), atg1Δ, or pep4Δ strains expressing Om45-GFP (A) or Idh1-GFP (B) were cultured in YPL medium to mid-log growth phase and then shifted to SD-N medium for 0, 4 and 6 h. GFP processing was monitored by immunoblotting with anti-GFP and anti-Pgk1 (loading control) antibody or antiserum, respectively. The positions of molecular mass markers are indicated on the right.