FIGURE 4.

Screening atg mutants for potential mitophagy defects during starvation. Strains deleted for the indicated ATG genes and expressing Om45-GFP were screened for potential mitophagy activity using the method demonstrated in Fig. 1. The atg mutant strains in A are either defective in all types of autophagy (atg8Δ and atg9Δ) or are defective in the Cvt pathway. The atg mutant strains in B are defective for all types of autophagy (atg1Δ) or are defective in nonspecific macroautophagy, but are normal for the Cvt pathway. The relative amount of processed GFP was calculated. The values represent the means and standard deviation from three independent experiments. WT, wild type.