FIGURE 6.

Analysis of pexophagy and the Cvt pathway. Wild-type (WT) and atg11Δ, atg20Δ, and atg24Δ (A) or atg17Δ, atg29Δ, and atg31Δ (B) strains expressing Pex14-GFP were cultured with oleic acid-containing medium for 19 h, then shifted to SD-N for the indicated times, and monitored for GFP processing by immunoblotting. The asterisks indicate nonspecific bands. The relative amount of processed GFP was calculated. The values represent the means and standard deviation from three independent experiments. C, each mutant was cultured in YPD medium and analyzed for prApe1 maturation by immunoblotting to monitor the Cvt pathway during vegetative growth. The positions of precursor and mature Ape1 are indicated.