FIGURE 1.

Expression of JIL-1 deletion constructs transgenically in a JIL-1 null mutant background. A, diagrams of the JIL-1 GFP- or CFP-tagged constructs analyzed. The region in the CTD where JIL-1^Su(var)3-1 alleles, resulting in COOH-terminally truncated proteins have been mapped (4), is indicated by a bracket. B, immunoblot labeled with JIL-1 antibody of protein extracts from wild type (wt) and from JIL-1 null larvae expressing GFP-JIL-1, CTD, and ΔCTD. Labeling with lamin antibody was used as a loading control. The relative migration of molecular size markers is indicated to the left of the immunoblot in kDa. C, polytene chromosome squash preparations from male JIL-1^z2/JIL-1^z2 third instar larval salivary glands expressing GFP-JIL-1, ΔCTD, or CTD. The left panel shows a squash preparation from a JIL-1 null mutant larvae for comparison. Protein localization (in green) was identified using either JIL-1 (anti-JIL-1) or GFP (anti-GFP) antibodies, whereas DNA (in blue or gray) was labeled by Hoechst. The male X chromosome is indicated by an X.