FIGURE 6.
GTPase activity of different constructs of AtNOA1 and magnesium dependence of the reaction. The amounts of phosphate released, expressed in nmol of GTP hydrolyzed/nmol of protein, are presented for reactions containing MgCl2 plus no protein (○) or 3 μm Δ101 (▪), of Δ101T327A (♦), of AtNOA1-(176-561) (CPG-CTD domains (▴)), of AtNOA1-(102-350) (ZBD-CPG domains (♦)), or Δ101 in the absence of MgCl2 (□). All reactions were conducted at 37 °C in the presence of 4 μCi of [γ-32P]GTP and 500 μm GTP. Data points with slightly negative values were set to zero.