Skip to main content
. 2008 Nov 21;283(47):32462–32470. doi: 10.1074/jbc.M801813200

FIGURE 2.

FIGURE 2.

Quantitative PCR analysis (A), PDX-1 immunoblot (B), and glucose-stimulated insulin secretion (C), in isolated islets from 8-week-old male floxed control (Cre-) and PANC PPARγ-/- mice (Cre+). A, quantitative PCR analysis of various genes from total RNA preparations of isolated islets. α-Tubulin and cyclophilin A (Cycl A) were used as internal controls. A representative gel is shown, and the graph contains the mean ± S.E. band intensities from the three separate experiments, with the Cre+ islets expressed as % intensity compared with the Cre- islets. *, p < 0.05. B, representative immunoblot for PDX-1 and β-actin from islet lysates of two control and two PANC PPARγ-/- mice. C, insulin secretion from freshly isolated Cre- (n = 4) and Cre+ (n = 3) islets stimulated for 1 h with 2.8 or 16.7 mm glucose expressed as percentage of total insulin content. Also shown is the fold increase of the insulin response at 16.7 mm glucose versus 2.8 mm glucose.