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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: J Immunol. 2008 Sep 1;181(5):3575–3585. doi: 10.4049/jimmunol.181.5.3575

FIGURE 5. Requirement for Rho activation in mAb 217-induced Akt and eIF4E phosphorylation.

FIGURE 5

A, 3T3TβRII cells were stimulated with mAb 217 (100 µg/ml) for the time indicated and total cell lysates immunoblotted for phospho-eIF4E. B, 3T3TβRII cells were pre-incubated with the indicated concentrations of C3 transferase for 48 h and then stimulated with mAb 217 for 30 min (upper panel). 3T3TβRII cells were transiently transfected with empty vector, dominant negative RhoA N19 or constitutively active RhoA V14. After 48 h, the cells were incubated in the presence or absence of mAb 217 for 30 min (lower panel). Total cell lysates were immunoblotted for phospho-Akt or phospho-eIF4E.