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. Author manuscript; available in PMC: 2009 Aug 13.
Published in final edited form as: J Neuroimmunol. 2008 Jun 27;199(1-2):94–103. doi: 10.1016/j.jneuroim.2008.05.011

Figure 2.

Figure 2

Effect of CCL2 on the level of p-MKK3/6 in hippocampal cultures. (A) Concentration-dependent effect of CCL2 (10 min exposure) on the level of p-MKK3/6. (B) Time-dependent effect of CCL2 (25 nM) on the level of p-MKK3/6. (C) Effect of PTX (100 ng/ml) on the CCL2-induced increases in the levels of p-MKK3/6. Representative immunoblots and a graph of mean values are shown for all studies. The methods were similar to the methods described in Figure 1 for p38 MAPK. Cultures were pretreated with PTX overnight. In C, data from CCL2-treated cultures were normalized to data from untreated control cultures; data from CCL2- and PTX-treated cultures was normalized to data from cultures treated with PTX but no CCL2.