Figure 4.

Full-length dystrophin expression in MDX1-injected muscles. Muscles were analyzed for dystrophin expression 2 wk after injection of MDX1 or control (CON) chimeraplasts. Dystrophin was immunoprecipitated from injected muscles, and subsequent immunoblot analysis revealed a single band corresponding to the molecular mass of full-length dystrophin (427 kDa) in MDX1-injected muscles; no band was seen in muscles injected with control chimeraplasts. A nonspecific band at 115 kDa was seen in both groups of muscles (as well as in uninjected mdx muscle and in C57 muscle, data not shown). Identical results were seen in three different experiments, representing the analysis of six MDX1-injected muscles and six control chimeraplast-injected muscles.