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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2008 May 28;45(7):943–949. doi: 10.1016/j.freeradbiomed.2008.05.015

Scheme I.

Scheme I

The representation of the method used to determine levels of MnTE-2-PyP5+ in plasma and organs. After the proteins were precipitated from plasma and tissue homogenates, the Zn salt was added along with sodium ascorbate into the supernatant to exchange Mn(II) by Zn(II). While the reduction of the Mn(III)P occurred instantaneously, one hour of incubation at 50 or 70 °C is needed to yield a complete Mn by Zn exchange for the HPLC/fluorescence measurement of ZnTE-2-PyP4+.