Figure 1. Purification of CeWRN-1.
(A) SDS–PAGE analysis of purified CeWRN-1. CeWRN-1 was overproduced in E. coli as a fusion with an N-terminal six-histidine tag. Purified six-His-tagged CeWRN-1 (1 μg in lane 2) was analyzed by 8% SDS—PAGE and stained with Coomassie Blue. Lane 1 contained molecular size markers. (B) Five representative fragments (of 27 identified in total) of purified CeWRN-1 analyzed by MALDI-TOF. Amino acid (AA) positions are as indicated, and their corresponding E values are listed.