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. Author manuscript; available in PMC: 2008 Nov 17.
Published in final edited form as: Biochemistry. 2008 Jun 18;47(28):7583–7593. doi: 10.1021/bi800197m

Figure 4. CeWRN-1 helicase activity on a Holliday junction.

Figure 4

(A) Helicase reactions (10 μL) were performed by incubating varying amounts of CeWRN-1 with 1 nM synthetic Holliday junction at 37 °C for 15 min. The reaction products were analyzed via 10% nondenaturing PAGE and were subjected to phosphorimaging analysis: lane 1, no enzyme control; and lane 8, heat-denatured DNA substrate control. Lanes 2–7 show a protein titration (1.25, 2.5, 5, 10, 20, and 40 nM, respectively). The substrate and product are indicated schematically at the right. (B) Quantification of the data shown in panel A. The relative amount of dissociated product is expressed as the percentage of total DNA. The dissociated products include a forked duplex [the products of branch migration (△)] and ssDNA (□). Background levels of dissociated species have been subtracted. Data represent the means of at least three independent experiments ± SD.

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