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. Author manuscript; available in PMC: 2008 Nov 17.
Published in final edited form as: Biochemistry. 2008 Jun 18;47(28):7583–7593. doi: 10.1021/bi800197m

Table 1.

Structures of DNA Substrates for Helicase Assays

Name Structure Name Structure
3′-overhang
(1+2)
graphic file with name nihms-65084-t0001.jpg 4bp bubble
(10+11)
graphic file with name nihms-65084-t0002.jpg
5′-overhang
(2+3)
graphic file with name nihms-65084-t0003.jpg 12bp bubble
(12+13)
graphic file with name nihms-65084-t0004.jpg
Blunt-end duplex
(4+5)
graphic file with name nihms-65084-t0005.jpg 21bp bubble
(14+15)
graphic file with name nihms-65084-t0006.jpg
forked duplex
(6+7)
graphic file with name nihms-65084-t0007.jpg 100bp M13mp 18
Partial duplex
graphic file with name nihms-65084-t0008.jpg
forked duplex
(8+9)
graphic file with name nihms-65084-t0009.jpg FLAP
(2+16+18)
graphic file with name nihms-65084-t0010.jpg
FLAP
(2+16+17)
graphic file with name nihms-65084-t0011.jpg Holliday-Junction
(19+20+21+22)
graphic file with name nihms-65084-t0012.jpg
Synthetic
Replication fork
(2+14+15+16)
graphic file with name nihms-65084-t0013.jpg

Radioactive label at 5′

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