Determinations of the effects of BSO treatments on the cytotoxicity of CDDP (A) and Cu (B), and rates of uptake of CDDP (C) and Cu (D), expression of hCtr1, ATP7A, and ATP7B by western blots (E) and hCtr1. For cytotoxicity assays, cells grown in 96-well plates (104 cells/well) were continuously exposed to various concentrations of copper and CDDP in the presence or absence of 100 μM BSO. After 72 hr incubation, cytotoxicity was measured by MTT assay. The IC50 value (μM) was calculated by the Hill plot method with linear regression. * p<0.01, significantly different from SR3A cells. #: p<0.01, significantly different from SR3A cell in the presence of BSO.