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. Author manuscript; available in PMC: 2009 Oct 25.
Published in final edited form as: Virology. 2008 Aug 30;380(2):276–284. doi: 10.1016/j.virol.2008.07.013

Fig. 1. Expression of wild type (WT) YFV NS5, empty vector control (EV) and their mass spectrometric (MS) analysis.

Fig. 1

(A) HEK293T cells were transfected with WT YFV NS5 and EV separately. After 48 hours of transfection the proteins were pull down by 6X His and identified in 10% SDS-PAGE gel with colloidal staining method. 100 kD band is correspond to WT YFV NS5 which is absent EV control lane. (B) MALDI-TOF full scan spectra correspond to 100 kD size band of WT YFV NS5 (*) and EV control lane (**). The EV MS data was used as a background control in each peptide analysis.