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. Author manuscript; available in PMC: 2009 Oct 25.
Published in final edited form as: Virology. 2008 Aug 30;380(2):276–284. doi: 10.1016/j.virol.2008.07.013

Fig. 7. In vitro methylating activity of YFV NS5s.

Fig. 7

Authentic YFV RNA corresponding to the first 250bp that had been capped by VP39 of Vaccinia virus as well as S-adenosyl methionine were incubated with either a mock (1), vaccinia 2-0 methyltransferase (2), the wild type YFV methyl transferase (3), and mutant YFV NS5s S56D (4) and S56A (5). This reaction was then run out by TLC and the ability to perform 2’-O methylation was compared (middle spot, m7GpppA).