FIG. 4.

Physical map of plasmid pBMB0228, harboring both cry55Aa2 and cry6Aa2. Genes are indicated by arrows. The filled arrows indicate the genes with known functions, and the open arrows indicate genes having unknown functions. The filled box indicates the position of the PCR fragment frag0228, while the open boxes indicate the predicted positions of dso (double-stranded origin) and sso (single-stranded origin). The rep14-3-like and repL-like genes are the putative replication initiator protein genes, while two mob-like genes contribute to the mobilization of this plasmid. R2 is a negative-regulator gene of cry6Aa2, referred to as orf2 by Yu et al. (30).